Proteins are precipitated from aquaous solutions by dissolving ammonium sulfate into the protein solution. The following table shows the weight (g) of ammonium sulfate to be added to one litre of solution to produce a desired change in the concentration (% saturation) of ammonium sulfate. Precipitation of proteins is conventionally carried out at 0°C to avoid possible denaturation of proteins, although the % saturation values shown in the table are based on the values at 25°C described by Green and Hughes in Methods Enzymol. 1, 67-90 (1955).
% | 10 | 15 | 20 | 25 | 30 | 33 | 35 | 40 | 45 | 50 | 55 | 60 | 65 | 70 | 75 | 80 | 85 | 90 | 95 | 100 |
0 | 56 | 84 | 114 | 144 | 176 | 196 | 209 | 243 | 277 | 313 | 351 | 390 | 430 | 472 | 516 | 561 | 610 | 662 | 713 | 767 |
10 | 28 | 57 | 86 | 118 | 137 | 150 | 183 | 216 | 251 | 288 | 326 | 365 | 406 | 449 | 494 | 540 | 592 | 640 | 694 | |
15 | 28 | 57 | 88 | 107 | 120 | 153 | 185 | 220 | 256 | 294 | 333 | 373 | 415 | 459 | 506 | 556 | 605 | 657 | ||
20 | 29 | 59 | 78 | 91 | 123 | 155 | 189 | 225 | 262 | 300 | 340 | 382 | 424 | 471 | 520 | 569 | 619 | |||
25 | 30 | 49 | 61 | 93 | 125 | 158 | 193 | 230 | 267 | 307 | 348 | 390 | 436 | 485 | 533 | 583 | ||||
30 | 19 | 30 | 62 | 94 | 127 | 162 | 198 | 235 | 273 | 314 | 356 | 401 | 449 | 496 | 546 | |||||
33 | 12 | 43 | 74 | 107 | 142 | 177 | 214 | 252 | 292 | 333 | 378 | 426 | 472 | 522 | ||||||
35 | 31 | 63 | 94 | 129 | 164 | 200 | 238 | 278 | 319 | 364 | 411 | 457 | 506 | |||||||
40 | 31 | 63 | 97 | 132 | 168 | 205 | 245 | 285 | 328 | 375 | 420 | 469 | ||||||||
45 | 32 | 65 | 99 | 134 | 171 | 210 | 250 | 293 | 339 | 383 | 431 | |||||||||
50 | 33 | 66 | 101 | 137 | 176 | 214 | 256 | 302 | 345 | 392 | ||||||||||
55 | 33 | 67 | 103 | 141 | 179 | 220 | 264 | 307 | 353 | |||||||||||
60 | 34 | 69 | 105 | 143 | 183 | 227 | 269 | 314 | ||||||||||||
65 | 34 | 70 | 107 | 147 | 190 | 232 | 275 | |||||||||||||
70 | 35 | 72 | 110 | 153 | 194 | 237 | ||||||||||||||
75 | 36 | 74 | 115 | 155 | 198 | |||||||||||||||
80 | 38 | 77 | 117 | 157 | ||||||||||||||||
85 | 39 | 77 | 118 | |||||||||||||||||
90 | 38 | 77 | ||||||||||||||||||
95 | 39 |
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