Prof. Kunio Miki


1952: Born in Kobe, Japan
1975: Bachelor of Engineering, Osaka University
1977: Master of Engineering, Osaka University
1981: Doctor of Engineering, Osaka University
1978-1990: Research Associate, Osaka University
1982-1983: Post-Doctoral Fellow, Max-Planck-Institute for Biochemistry, Martinsried near Munich, Germany
1991-1994: Associate Professor, Tokyo Institute of Technology
1994-present: Professor, Kyoto University
1999-present: Chief Scientist, Director of Laboratory, RIKEN Harima Institute / SPring-8


Selected Publications

For more detail publication list, please refer to "Publications" in the Home page of the laboratory.


"Structural Insight into Gene Duplication, Gene Fusion and Domain Swapping in the Evolution of PLP-independent Amino Acid Racemases"
L. Liu, K. Iwata, M. Yohda, and K. Miki
FEBS Lett., in press (2002).

"Ultrahigh-Resolution Structure of High-Potential Iron-Sulfur Protein from Thermochromatium tepidum"
L. Liu, T. Nogi, M. Kobayashi, T. Nozawa, and K. Miki
Acta Crystallogr., Sect. D, 58, 1085-1091 (2002).

"Crystal Structure of Aspartate Racemase from Pyrococcus horikoshii OT3 and Its Implication for Molecular Mechanism of PLP-independent Racemization"
L. Liu, K. Iwata, A. Kita, Y. Kawarabayasi, M. Yohda, and K. Miki
J. Mol. Biol., 319, 479-489 (2002).

"Crystal Structure of the Complex between Calyculin A and the Catalytic Subunit of Protein Phosphatase 1"
A. Kita, S. Matsunaga, A. Takai, H. Kataiwa, T. Wakimoto, N. Fusetani, M. Isobe, and K. Miki
Structure, 10, 715-724 (2002).

"Crystal Structure of Thermostable DNA Photolyase: Pyrimidine-Dimer Recognition Mechanism"
H. Komori, R. Masui, S. Kuramitsu, S. Yokoyama, T. Shibata, Y. Inoue, and K. Miki
Proc. Natl. Acad. Sci. USA, 98, 13560-13565 (2001).

"Crystal Structure of Chaperonin-60 from Paracoccus denitrificans"
T. A. Fukami, M. Yohda, H. Taguchi, M. Yoshida, and K. Miki
J. Mol. Biol., 312, 501-509 (2001).

"Structure of a Novel-type Archaeal Rubisco with Pentagonal Symmetry"
K. Kitano, N. Maeda, T. Fukui, H. Atomi, T. Imanaka and K. Miki
Structure, 9, 473-481 (2001).

"Cytochrome c2"
K. Miki and S. Sogabe
"Handbook of Metalloproteins", A. Messerschmidt, R. Huber, T. L. Poulos and K. Wieghardt eds., John Wiley, Chichester, pp. 55-68 (2001).

"Crystal Structure of a Mutant Human Lysozyme with a Substituted Disulfide Bond"
K. Inaka, E. Kanaya, M. Kikuchi, and K. Miki
Proteins: Struct., Funct., Genet., 43, 413-419 (2001).

"Structure of the H Subunit of the Photosynthetic Reaction Center from the Thermophilic Purple Sulfur Bacterium, Thermochromatium tepidum: Implications for the Specific Binding of the Lipid Molecule to the Membrane Protein Complex"
I. Fathir, T. Mori, T. Nogi, M. Kobayashi, K. Miki and T. Nozawa
Eur. J. Biochem., 268, 2652-2657 (2001).

"Structural Basis of Bacterial Photosynthetic Reaction Centers"
T. Nogi and K. Miki
J. Biochem., 130, 319-329 (2001).

"Crystal Structure of cis-Prenyl Chain Elongating Enzyme, Undecaprenyl Diphosphate Synthase"
M. Fujihashi, Y.-W. Zhang, Y. Higuchi, X.-Y. Li, T. Koyama, and K. Miki
Proc. Natl. Acad. Sci. USA, 98, 4337-4342 (2001).

"Crystal Structure of the Oxidized Cytochrome c2 from Blastochloris viridis"
S. Sogabe and K. Miki
FEBS Lett., 491, 174-179 (2001).

"Structural Genomics Projects in Japan"
S. Yokoyama, H. Hirota, T. Kigawa, T. Yabuki, M. Shirouzu, T. Terada, Y. Ito, Y. Nishimura, Y. Kyogoku, K. Miki, R. Masui, and S. Kuramitsu
Nature Struct. Biol., 7, 943-945 (2000).

"Crystal Structures of Photosynthetic Reaction Center and High-Potential Iron-Sulfur Protein from Thermochromatium tepidum; Thermostability and Electron Transfer"
T. Nogi, I. Fathir, M. Kobayashi, T. Nozawa, and K. Miki
Proc. Natl. Acad. Sci. USA, 97, 13561-13566 (2000).

"Utilization of Microgravity to Improve Crystal Quality of Biologically Important Proteins"
K. Kitano, R. Sasaki, T. Nogi, T. A. Fukami, A. Nakagawa, K. Miki, and I. Tanaka
J. Cryst. Growth, 210, 819-823 (2000).

"Crystal Structure of Chitosanase from Bacillus circulans MH-K1 at 1.6 A Resolution and Its Substrate Recognition Mechanism"
J. Saito, A. Kita, Y. Higuchi, Y. Nagata, A. Ando, and K. Miki
J. Biol. Chem., 274, 30818-30825 (1999).

"Ribulose Bisphosphate Carboxylase/Oxygenase from the Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1 is Composed Solely of Large Subunits and Forms a Pentagonal Structure"
N. Maeda, K. Kitano, T. Fukui, S. Ezaki, H. Atomi, K. Miki, and T. Imanaka
J. Mol. Biol., 293, 57-66 (1999).

"Crystal Structure of a Prokaryotic Replication Initiator Protein Bound to DNA at 2.6 A Resolution"
H. Komori, F. Matsunaga, Y. Higuchi, M. Ishiai, C. Wada, and K. Miki
EMBO J., 18, 4597-4607 (1999).

"An Archetypical Extradiol-Cleaving Catecholic Dioxygenase: The Crystal Structure of Catechol 2,3-Dioxygenase (Metapyrocatechase)"
A. Kita, S. Kita, I. Fujisawa, K. Inaka, T. Ishida, K. Horiike, M. Nozaki, and K. Miki
Structure, 7, 25-34 (1999).

"A Novel Approach to Crystallizing Proteins with Temperature Induction Method: GrpE Protein from Thermus thermophilus"
K. Kitano, K. Motohashi, M. Yoshida, and K. Miki
J. Cryst. Growth, 186, 456-460 (1998).

"Crystal Structure of DNA Photolyase from Anacystis nidulans"
T. Tamada, K. Kitadokoro, Y. Higuchi, K. Inaka, A. Yasui, P. E. de Ruiter, A. P. M. Eker, and K. Miki
Nature Struct. Biol., 4, 887-891 (1997).

"Electrostatic Properties Deduced from Refined Structures of NADH-Cytochrome b5 Reductase and the Other Flavin-Dependent Reductases: Pyridine Nucleotide-Binding and Interaction with an Electron-Transfer Partner"
H. Nishida and K. Miki
Proteins: Struct., Funct., Genet., 26, 32-41 (1996).

"The Structure of Flavoprotein FP390 from a Luminescent Bacterium, Photobacterium phosphoreum at 2.7 A Resolution"
A. Kita, S. Kasai, M. Miyata, and K. Miki
Acta Crystallogr., Sect. D, 52, 77-86 (1996).

"Refined Crystal Structure of Ferrocytochrome c2 from Rhodopseudomonas viridis at 1.6 A Resolution"
S. Sogabe and K. Miki
J. Mol. Biol., 252, 235-247 (1995).

"Crystal Structure of a Gultathionylated Mutant Human Lysozyme: A Folding Intermediate Mimic in the Formation of a Disulfide Bond"
K. Inaka, M. Kikuchi, K. Miki, Y. Taniyama, and M. Matsushima
Acta Crystallogr., Sect. D, 51, 619-625 (1995).

"Crystal Structure of NADH-Cytochrome b5 Reductase from Pig Liver at 2.4 A Resolution"
H. Nishida, K. Inaka, M. Yamanaka, S. Kaida, K. Kobayashi, and K. Miki
Biochemistry, 34, 2763-2767 (1995).

"Fundamental Design of the High Energy Undulator Pilot Beamline for Macromolecular Crystallography at SPring-8"
N. Kamiya, T. Uraga, H. Kimura, H. Yamaoka, M. Yamamoto, Y. Kawano, T. Ishikawa, H. Kitamura, T. Ueki, Y. Kashihara, N. Tanaka, H. Moriyama, K. Hamada, K. Miki, and I. Tanaka
Rev. Sci. Instrum., 66, 1703-1705 (1995).

"Structural Similarity of Cytochrome c2 from Rhodopseudomonas viridis to Mitochondrial Cytochromes c Revealed by Its Crystal Structure at 2.7 A Resolution"
S. Sogabe, T. Ezoe, N. Kasai, M. Saeda, A. Uno, M. Miki, and K. Miki
FEBS Lett., 345, 5-8 (1994).


E-mail: miki @ kuchem. kyoto-u. ac. jp